Sau-PCR, a Novel Amplification Technique for Genetic Fingerprinting of Microorganisms

DNA Fingerprinting by PCR Amplification of HLA Genes

A PCR fingerprinting technique to distinguish isolates of Lactococcus lactis.

A fingerprinting technique similar to repetitive extragenic palindromic PCR was developed to identify strains of Lactococcus lactis. The method distinguishes closely related strains and discriminates among some with identical ldh sequences. The fingerprinting primer LL-Rep1 complements a moderately repeated sequence found in low G + C Gram-positive bacteria and may therefore prove useful for di...

A Novel Technique for Steganography Method Based on Improved Genetic Algorithm Optimization in Spatial Domain

This paper devotes itself to the study of secret message delivery using cover image and introduces a novel steganographic technique based on genetic algorithm to find a near-optimum structure for the pair-wise least-significant-bit (LSB) matching scheme. A survey of the related literatures shows that the LSB matching method developed by Mielikainen, employs a binary function to reduce the numbe...

Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification technique

Mapping sites within the genome that are hypersensitive to digestion with DNaseI is an important method for identifying DNA elements that regulate transcription. The standard approach to locating these DNaseI-hypersensitive sites (DHSs) has been to use Southern blotting techniques, although we, and others, have recently published alternative methods using a range of technologies including high-...

Optimization of the Aflp-fingerprinting Method for a Molecular-genetic Certification of Agricultural Microorganisms

The study involved 16 strains of the Russian collection of agricultural microorganisms (RCAM) related to the genera Bacillus, Lactobacillus, Rhizobium, and Bradyrhizobium. The aim of the work was to develop a procedure of the genotypic certification of strains with AFLP-fingerprinting (amplified fragment length polymorphism), providing detection of nucleotide polymorphisms and small rearrangeme...